Fig. 8.

In the presence of suramin (10 μM) and α,β-methylene-ATP (10 μM), BRL37344 (100 μM) decreased the amplitude of the EFS-induced (0.5–50 Hz) contractions in DSM isolated strips. A, C: original EFS-induced contraction recordings showing BRL37344 (100 μM) inhibitory effect in the absence (A) or presence (C) of SR59230A (10 μM). B, D: frequency-response curves showing BRL37344 inhibitory effect on EFS-induced contraction amplitude in the absence (B; n = 10, N = 5, **P < 0.01, ***P < 0.005) or presence (D) of 10 μM SR59230A (n = 10, N = 5, **P < 0.01, ***P < 0.005). EFS-induced contraction amplitude at 50 Hz under control conditions was taken to be 100%.