Table 1.
Summarized data illustrating a lack of effect of BRL37344 (100 μM) on cell membrane excitability in guinea pig DSM freshly isolated cells
| Control | BRL37344 (100 μM) | Statistics | ||
|---|---|---|---|---|
| TBKCs | Amplitude −40 mV | 24.0 ± 4.6 pA | 26.3 ± 6.5 pA | n = 9; N = 7; P > 0.05 |
| Amplitude −20 mV | 19.8 ± 2.4 pA | 18.16 ± 1.1 pA | n = 6; N = 5; P > 0.05 | |
| Frequency −40 mV | 50.2 ± 22.8 TBKCs/min | 65.76 ± 30.4 TBKCs/min | n = 9; N = 7; P > 0.05 | |
| Frequency −20 mV | 45.3 ± 18.2 TBKCs/min | 74.23 ± 31.2 TBKCs/min | n = 6; N = 5; P > 0.05 | |
| Voltage step-induced whole cell current | 0 mV | 2.3 ± 1.0 pA/pF | 1.1 ± 0.3 pA/pF | n = 4; N = 2; P > 0.05 |
| 20 mV | 3.5 ± 1.1 pA/pF | 1.8 ± 0.2 pA/pF | n = 4; N = 2; P > 0.05 | |
| 40 mV | 5.9 ± 1.7 pA/pF | 3.6 ± 0.6 pA/pF | n = 4; N = 2; P > 0.05 | |
| 60 mV | 11.4 ± 3.3 pA/pF | 10.8 ± 1.9 pA/pF | n = 4; N = 2; P > 0.05 | |
| 80 mV | 22.8 ± 7.5 pA/pF | 22.7 ± 5.1 pA/pF | n = 8; N = 4; P > 0.05 | |
| Resting membrane potential recorded in current-clamp mode | −22.8 ± 3.5 mV | −21.6 ± 4.0 mV |
Values are means ± SE. Perforated whole cell patch-clamp experiments revealed no effect of 100 μM BRL37344 on transient BK currents (TBKCs) amplitude and frequency recorded at −40 and −20 mV. Whole cell outward K+ current amplitude was also unchanged after application of 100 μM BRL37344. Current-clamp experiments revealed that 100 μM BRL37344 did not significantly change the level of the resting membrane potential in guinea pig detrusor smooth muscle (DSM) freshly isolated cells.