Solution 31P NMR spectra of NaOH–EDTA
extracts
of a soil (East 50) from the Falkland Islands before (A) and after
(B) pretreatment with hypobromite oxidation to destroy all organic
phosphorus other than the higher-order inositol phosphates. The zoomed
insets show the phosphonate signals between δ = 19 and 21 ppm.
Spectra are plotted with 5 Hz line broadening. In (B) the spectrum
is truncated vertically to show the scyllo-inositol
hexakisphosphate signal at the same height in both spectra. Signal
assignments are as follows: a, neo- and d-chiro-inositol hexakisphosphate; b, inorganic orthophosphate;
c, phosphomonoesters other than the signals from neo- and d-chiro-inositol hexakisphosphate
between δ = 6.4 and 6.9 ppm; d, alkali-stable phospholipids;
e, DNA; f, pyrophosphate; g, phosphonates.