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. 2012 Apr 2;109(16):6012-6017. doi: 10.1073/pnas.1118440109

Fig. 2.

Fig. 2.

Cellulase expression levels in WT and β-glucosidase deletion strains after induction with cellobiose or Avicel. (A) Gene expression of select cellulases after 4 h induction with 0.2% cellobiose or 1% Avicel in WT, Δ3βG, and Δ3βGΔcre strains. Gene expression levels of cbh-1, gh6-2, and gh5-1 were normalized to 1 when induced with 1% sucrose. Expression levels of actin (NCU04173) were used as an endogenous control in all samples. Each strain was grown in triplicate and error bars indicate 1 SD. (B) SDS-PAGE of secreted proteins in culture filtrates from WT, Δ3βG, and Δ3βGΔcre strains. Growth and induction conditions are described in Materials and Methods. Protein bands representing CBH-1, GH6-2, and GH5-1 are marked. In addition, the absence of the extracellular β-glucosidase (NCU04952) is marked in the triple knockout. The presence of glucoamylase I (NCU01517) correlates with the deletion of the cre-1 gene (21). (C) Activity of supernatant from B toward Avicel. Glucose (dark gray) and cellobiose (light gray) were measured after 24 h of incubation with 1% Avicel at 50 °C. Error bars are 1 SD.