Table 1.
Mass spectrometry of the most abundant secreted proteins in N. crassa WT (Avicel), Δ3βG (cellobiose), and Δ3βGΔcre (cellobiose) strains
| Gene | Annotation | WT | Δ3βG | Δ3βGΔcre | Secretome (%)* |
| Cellulases | |||||
| NCU07340 | CBH-1 | + | + | + | 39.5 |
| NCU09680 | GH6-2 | + | + | + | 13.4 |
| NCU07898 | GH61-2 | + | — | + | 6.6 |
| NCU00762 | GH5-1 | + | + | + | 5.9 |
| NCU08760 | GH61-5 | + | + | + | 4.6 |
| NCU05057 | GH7-1 | + | + | + | 4.0 |
| NCU02240 | GH61-1 | + | — | + | 3.4 |
| NCU07190 | GH6-3 | + | + | + | 3.2 |
| Accessory proteins | |||||
| NCU04952 | GH3-4 | + | ND | ND | 3.8 |
| NCU00206 | CDH-1 | + | + | + | 2.4 |
| NCU09764 | N/A | + | + | + | 1.6 |
| NCU05137 | NCW-1 | + | + | + | 1.5 |
| NCU07143 | LAC-2 | + | + | + | 1.0 |
GH, glycoside hydrolase; ND, not detected, gene knockout
*Avicel-induced secretome identified by AQUA Mass Spectrometry, Phillips, et al. (35) (13 proteins represent 91% of the total secretome with all other proteins representing less than 1% of the secretome each).