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. 2012 Mar 28;109(16):E981–E988. doi: 10.1073/pnas.1120742109

Fig. 1.

Fig. 1.

Loss of DDM1 activity increases meiotic recombination rates in heterozygote DDM1/ddm1-2 and homozygote ddm1-2/ddm1-2 plants. Recombination rates of DDM1/ddm1-2 were obtained from F2 seeds of a cross between the ddm1-2 mutant and the tester line Col3-4/20, which contained green and red fluorescent markers. The seeds used to monitor recombination rates of ddm1-2/ddm1-2 were obtained from the introgression of the fluorescent seed markers from tester line Col3-4/20 into the ddm1-2 mutant background. The homozygous ddm1-2 point mutation was confirmed by sequencing. WT represents seeds from crosses between tester line Col3-4/20 and WT plants. N = number of seeds obtained from five to nine plants) scored in each background. The variation in recombination between individual plants was used to determine the SE (shown as error bars).