Fig. 3.

CLIC4 and iNOS knockout macrophages exhibit similar dysregulation of IL-1β and CXCL1 but not TNFα mRNA after prolonged stimulation with LPS/IFNγ. Primary mouse macrophages from CLIC4 knockout, iNOS knockout, and wild-type mice were treated with LPS and IFNγ (1 μg/mL and 10 ng/mL, respectively) for the indicated times. Lysates were used for real-time PCR analysis of IL-1β, CXCL1, and TNFα mRNA. Bars represent the mean ± SEM of three replicates. Statistical significance was determined using a two-tailed unpaired Student's t test and is indicated.