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Urea-SDS-PAGE analysis of PSII samples used for reaction-induced FT-IR spectroscopy. After three resuspension steps, samples were treated with 100 mM NaCl or NH4Cl at pH 6.0 or 7.5 for 1 h (0 °C). Control (Ctrl) samples contained only 15 mM NaCl. PSII samples were isolated by the procedure of (19). A photosystem II membrane preparation (BBY) was used as a control (18). The protein standards are labeled with molecular weights (kDa) on the left.
