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Overview of the biotinyl-lysine reaction scheme for detection of sirtuin-mediated deacetylation. (I) Lysines and N termini are acetylated with acetic anhydride. (II) Sirtuin-mediated NAD+-dependent deacetylation removes acetyl groups to regenerate unmodified lysines. (III) Deacetylated lysines are biotinylated with sulfo-NHS-biotin.
