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Schematic depicting changes in molecular weight and mass/charge peak shifts in MALDI-TOF as a result of deacetylation and biotinylation. The molecular weight of a peptide including a lysine is increased by 42 Da by acetylation. Deacetylation removes this 42 Da modification, and biotinylation adds 226 Da. Deacetylated, biotinylated lysines therefore result in a net change of 184 Da compared to acetylated lysines.
