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Sample MALDI mass spectrum of Hst2-deacetylated histones. (A) MALDI-TOF spectrum of histone peptide peaks in a control reaction lacking NAD+. (B) MALDI mass spectrum of a deacetylation reaction containing Hst2, NAD+, and histones shows mass shifting of peptides that are deacetylated and biotinylated in comparison to A.
