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. 2012 Mar 26;109(16):E926–E933. doi: 10.1073/pnas.1106300109

Fig. 1.

Fig. 1.

Low-dose 137Cs γ rays up-regulate TCTP and induce protective effects. (A) MN formation in AG1522 cells exposed to no or 10-cGy radiation delivered at a low dose rate (0.2 cGy/h). Control and irradiated confluent cells were subcultured, and MN formation was evaluated in binucleated cells following 72 h incubation. (B) Western blot analyses in confluent AG1522 cells exposed to no or 10-cGy radiation (0.2 cGy/h) and harvested for analysis within 5 min after irradiation. (C) Western blot analyses of TCTP in nuclei of AG1522 confluent cell cultures exposed to no or to 10-cGy radiation at an acute dose rate (6 cGy/min). TBP was used as the loading control. (D) Western blot analysis of TCTP in AG1522 confluent cell cultures exposed to no or to 10-cGy radiation at an acute dose rate (6 cGy/min) in the presence (+) or absence (−) of cycloheximide (CH). Cells were incubated in cycloheximide 30 min before irradiation and were harvested for analysis 4 h later.