Fig. 3.

Effects of ATM, DNA-PK, or ATR signaling in the regulation of TCTP levels in γ-irradiated confluent human cells. (A) Immunoblotting of ATM-mutated cells shows lack of TCTP induction by low-dose γ rays. (B) Immunoblots of nuclear extracts of AG1522 fibroblasts exposed to no or 10-cGy radiation in the absence (“C”) or presence of Ku55933 (10 μM), a selective inhibitor of ATM. (C) Immunoblot analysis in nuclear extracts from cells that are WT or mutated for DNA-PKcs. (D) Immunoblots of nuclear extracts from AG1522 cells irradiated in the presence of NU7441, a selective inhibitor of DNA-PK. (E) Immunoblots of nuclear extracts from nonirradiated and irradiated AG1522 cells transfected with Scr siRNA or siRNAs targeting ATM, ATR, or DNA-PKcs.