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. 2012 Mar 26;109(16):E926–E933. doi: 10.1073/pnas.1106300109

Fig. 4.

Fig. 4.

TCTP interacts with components of DNA damage sensing and repair. (A) Benzonase-treated chromatin-enriched fractions that were isolated from confluent U2OS cells 30 min after no (−) or acute [50 cGy, (+)] irradiation (IR) were immunoprecipitated with anti-ATM or anti-TCTP antibodies or normal mouse or rabbit serum (PI). Immunoblots then were reacted with antibodies against ATM, TCTP, γH2A.X, or H2A. X. (B) Benzonase-treated nuclear extracts isolated 30 min after exposure of U2OS confluent cells to no or acute (50 cGy) irradiation were immunoprecipitated with anti-TCTP, anti-p53, or control anti-TBP antibodies. Mouse or rabbit preimmune serum (PI) was used as a control. Immunoblotting was performed using antibodies against p53, TCTP, or TBP. (C) Immunoblotting of TCTP, Ku70, and Ku80 in Benzonase-treated nuclear extracts of control nonirradiated U2OS confluent cells after IP with either normal serum (PI) or antibodies against TCTP, Ku70, or Ku80. (D) Untreated or γ-irradiated (acute, 100 cGy) AG1522 asynchronous cells were preextracted, fixed 1 h later, and immunostained in situ with anti-TCTP, anti–P-ATM (S1981), anti-γH2A.X, or anti-53BP1 antibodies. (Scale bars, 10 μm.) (E) Quantitative assessment of colocalization of TCTP foci with those of P-ATM (S1981) (Left), γH2A.X (Center), and 53BP1 (Right) in AG1522 asynchronous cells at 1 h after exposure to 50, 100, or 200 cGy.