Fig. 2.

Characterization of protein isoforms using the single-microchannel 80-min LAVAgel immunoblot. Fluorescence micrographs show (A) loading and IEF of a CE540-labeled protein ladder with 617-nM green WT GFP, and (B) IEF readout via UV excitation. (C) After UV gel photoactivation, the pH gradient is washed out with retention of a portion of each WT GFP isoform. (D and E) Antibody probing of WT GFP with 100 nM Texas red-labeled polyclonal antibody (pAb*) demonstrates specificity and low-background. RFU, relative fluorescence units; CytC, cytochrome C; LCL, lentil lectin; Mb, myoglobin; CA, carbonic anhydrase; BLG, β-lactoglobulin; GOx, glucose oxidase.