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. 2012 Apr 2;109(16):5972-5977. doi: 10.1073/pnas.1108617109

Fig. 4.

Fig. 4.

LAVAgel assay enables quantitation of PSA isoforms in minimally processed prostate cancer cell lysate and human sera. (A) Fluorescence micrographs and electropherograms for probing of unlabeled PSA purified from human seminal fluid (500 nM): focused pI markers, primary (1°), and secondary (2°) antibody probe signals. Bracketed peak areas used to construct calibration curves. (B) Linear PSA calibration curves for primary (black circles) and secondary (red squares) antibody readouts (RFU, relative fluorescence units; ± SD, n = 4 for all points except 5 nM, n = 2). (C) Primary antibody probing of endogenous PSA isoforms in lysate from a PSA-producing cell line (LAPC-4 cells, +) with negative control lysate (DU145 cells, −). (D) Serum samples from metastatic prostate cancer patients probed with primary antibody to PSA (patients 1 and 2), alongside a low-PSA negative control serum (−).