Fig. 6.

IL-15 combined with blockade of CTLA-4 and PD-L1 decreased the suppressor function on a per-cell basis. (A) CD8⁺CD122⁺ T cells derived from treated tumor-bearing mice were cultured with CFSE-labeled naïve Ly-5.1⁺ CD8⁺CD122⁻ T cells and stimulated with plate-bound anti-CD3 plus soluble anti-CD28 for 48 h. Cells were gated on Ly5.1⁺ T cells to detect CFSE dilution. CD8⁺CD122⁻ T cells cultured alone were used as control. (B) Percentage suppression was enumerated. Data represent four independent experiments. n = 8–10 mice. *P < 0.05.