Fig. 4.

Total amount of NSP4 in PMNs is 20-fold lower than that of CG; nevertheless, NSP4 is detected in supernatants of stimulated PMNs. (A) NSP4 and CG in total cell lysates of PMNs were compared with known amounts of purified CG and NSP4 by semiquantitative Western blotting using monospecific anti-CG antibodies and anti-NSP4 mAbs, respectively. Content of NSP4 in PMNs is given as a percentage of CG present in PMNs. Data were pooled from three independent experiments (n = 3, ± SD). (B) PMNs were incubated at 37 °C in the presence or absence of 5 μg/mL cytochalasin B (Cyt B) and 200 ng/mL PMA for 30 min. Cell-free supernatants were analyzed by Western blotting with anti-NSP4 mAbs.