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(A) In vitro assay for the synthesis of acetoacetyl-CoA using crude extracts of wild-type S. elongatus PCC 7942, strain EL14 and EL20. (B) In vitro assay for the thiolysis of acetoacetyl-CoA using crude extracts of wild-type S. elongatus PCC 7942, strain EL14 and EL20. (C) Cell density and (D) 1-butanol accumulation as a function of time of strain EL14 and EL20. 1-Butanol production by strain EL14 was near detection limit of about 1 mg/L.
