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. 2012 Apr 30;197(3):421–437. doi: 10.1083/jcb.201108143

Figure 1.

Figure 1.

TGF-β treatment of MCF10A cells results in Hic-5–dependent increased matrix degradation, motility, and invasion. (A) Western blot analysis of MCF10A cells treated with vehicle and TGF-β. TGF-β–treated cells have increased Hic-5 and α smooth muscle actin protein expression. Up-regulated Hic-5 is efficiently depleted using two independent human-specific Hic-5 siRNAs (hHic-5). Paxillin is efficiently depleted using two independent siRNAs. Molecular mass standards are indicated next to the gel blots in kilodaltons. (B) Vehicle and TGF-β–treated MCF10A cells plated on fluorescent 488–gelatin. TGF-β–treated cells demonstrate increased degradation of underlying matrix, which is reduced with Hic-5 RNAi but not paxillin RNAi. Bars: (main panels) 20 µm; (insets) 4 µm. (C) Quantitation of cells associated with matrix degradation. (D) Quantitation of the area of matrix degraded per cell area. **, P < 0.005; ***, P < 0.0005. Breaks in the x axis indicate separate sets of experiments, each n = 3. Error bars represent the standard error of the mean.