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. 2012 Apr 30;197(3):421–437. doi: 10.1083/jcb.201108143

Figure 2.

Figure 2.

Hic-5 localizes to invadopodia in TGF-β–treated MCF10A cells. (A) TGF-β–treated MCF10A cells plated on fluorescent gelatin. Endogenous Hic-5 and paxillin colocalize in a ring around areas of matrix degradation, and actin and cortactin localize to the core structure. TGF-β–treated MCF10A cells plated on collagen show the focal adhesion protein actopaxin localized in a ring around an actin- and cortactin-containing core. Representative line profile plots demonstrate the distribution of Hic-5, paxillin, and actopaxin to the ring and actin, and cortactin to the core of invadopodia. Bars: (main panels) 10 µm; (insets) 1 µm. (B) TGF-β–treated cells demonstrate increased migration toward serum in a modified Boyden chamber assay. Hic-5 knockdown results in decreased migration. (C) TGF-β–treated cells have enhanced ability to invade through Matrigel, which is reduced with Hic-5 knockdown. *, P < 0.05; **, P < 0.005; ***, P < 0.0005. Breaks in the x axis indicate separate sets of experiments, each n = 3. Error bars represent the standard error of the mean.