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. 2012 Apr 30;197(3):391–405. doi: 10.1083/jcb.201106101

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© 2012 Inoko et al.

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Figure 5. — Knockdown of trichoplein causes a cilium-dependent cell cycle arrest in RPE1 cells. (A–D) Proliferating RPE1 cells were transfected with the combination of each siRNA and then subjected to FACS analysis (A and B), immunoblotting (C, top), or immunofluorescence (D). We used IFT-20 Sq. 1 in A–D; we also obtained similar results using another IFT-20 target sequence (IFT-20 Sq. 2; Fig. S2). (C, bottom) The percentage of cells with primary cilia was calculated as described in the legend to Fig. 2 B. Data are means ± SD. (D) Insets indicate higher magnifications. siCont., control siRNA; siTricho., trichoplein siRNA; Ac-Tub., acylated tubulin. Bars: (D, insets) 1 µm; (D, main images) 10 µm.