Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Apr 30;197(3):391–405. doi: 10.1083/jcb.201106101

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Inoko et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 6. — Trichoplein binds and activates AurA. (A) AurA can bind trichoplein but not Odf2-β in HeLa cells. The cells were transfected with the indicated proteins. 24 h after the transfection, cell extracts were subjected to immunoprecipitation with anti-Flag. Before immunoprecipitation, a fraction of each cell extract was subjected to immunoblotting with anti-MBP (trichoplein) or anti-GST (Odf2-β) as an input. (B) AurA can be activated by trichoplein in HeLa cells. The cells were transfected with Flag-tagged AurA wild type or its kinase-dead mutant (KM) in addition to Myc-tagged trichoplein and/or HA-tagged Odf2-β. 24 h after transfection, cell lysates were subjected to immunoblotting with anti-pAurA, anti-Flag (AurA), anti-Myc (trichoplein), or anti-HA (Odf2-β). (C) AurA can directly bind trichoplein in vitro. GST pull-down assays were performed using the indicated purified proteins. GST or MBP was used as a negative control (−). (D) AurA can be directly activated by trichoplein in vitro. The kinase assays were performed as described in Materials and methods. Each reaction mixture was subjected to autoradiography (³²P-H3 or ³²P-AurA) or immunoblotting with anti-GST (AurA) or anti-MBP (MBP or MBP-trichoplein). CBB, Coomassie brilliant blue; IP, immunoprecipitation; Tricho., trichoplein; IB, immunoblot.