Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Apr 30;197(3):439–455. doi: 10.1083/jcb.201201124

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 3. — Nonpolarized PIP3, Rac1, and Cdc42 signaling during lobopodia-based 3D migration. (A) Maximally projected confocal stacks of HFFs expressing GFP-AktPH to detect PIP3, migrating on the 2D CDM, in the 3D CDM, or inside 1.7 mg/ml of 3D collagen. Images were pseudocolored according to the 16-color scale. Bars, 10 µm. (B) Mean PI of GFP-AktPH in HFFs migrating in the indicated ECM environments. (C–F) Localization of active Rac1 and Cdc42 away from the leading edge during lobopodia-based 3D migration inside the CDM. (C and E) Maximally projected confocal stacks of HFFs expressing Rac1 (C) or Cdc42 (E) biosensors migrating on the 2D CDM (left), in the 3D CDM (middle), or in 3D collagen (right). The Fc images, representing the total activity of each GTPase, were pseudocolored according to the 16-color scale. Arrowheads indicate regions of intracellular signaling. Bars: (3D CDM) 5 µm; (collagen and 2D CDM) 10 µm. (D and F) Mean PI of active Rac1 (D) and active Cdc42 (F) in HFFs migrating in the indicated ECM environments. All cells are oriented with the leading edge toward the top of the figure. Error bars show means ± SEM. *, P < 0.05 versus the 2D CDM; **, P < 0.05 versus the 3D CDM.