Table 1. AdoMetDC activity, AdoMet and dcAdoMet content in TPA-treated wild type and CAG-SpmS mice.
| Tissue | Group | Time after TPA (h) | AdoMetDC activity (pmol CO2/30 min/mg protein) |
AdoMet (pmol/mg tissue) |
dcAdoMet (pmol/mg tissue) |
|---|---|---|---|---|---|
| Epidermis |
Wild type |
0 |
49 ± 24 |
7.3 ± 2.0 |
0.081 ± 0.012 |
| |
24 |
202 ± 158e |
18.0 ± 3.9h |
0.321 ± 0.288 |
|
| |
|
48 |
424 ± 237g |
20.6 ± 10.5f |
0.335 ± 0.190g |
| |
CAG-SpmS |
0 |
75 ± 18a |
11.9 ± 3.2a |
0.048 ± 0.036a |
| |
|
24 |
142 ± 65e |
20.5 ± 6.9 |
0.054 ± 0.035a |
| |
|
48 |
281 ± 118g |
18.4 ± 4.9 |
0.087 ± 0.041c |
| |
|
|
|
|
|
| Dermis |
Wild type |
0 |
44 ± 22 |
7.2 ± 2.1 |
0.103 ± 0.033 |
| |
|
24 |
99 ± 18h |
10.2 ± 2.3e |
0.082 ± 0.014 |
| |
|
48 |
129 ± 76e |
11.3 ± 2.9g |
0.087 ± 0.020 |
| |
CAG-SpmS |
0 |
44 ± 12 |
6.7 ± 3.1 |
0.095 ± 0.028 |
| |
|
24 |
76 ± 10b,h |
10.7 ± 1.4 |
0.018 ± 0.004d,g |
| 48 | 90 ± 43e | 9.2 ± 3.2 | 0.017 ± 0.007c,e |
AdoMetDC enzymatic activity as well as AdoMet and dcAdoMet content were determined in 7 to 9-week-old mice. Values represent mean ± SD of 4 to 8 mice. ap < 0.05 vs. wild type at the same time; bp < 0.01 vs. wild type at the same time; cp < 0.005 vs. wild type at the same time; dp < 0.001 vs. wild type at the same time; ep < 0.05 vs. 0h of the same genotype; fp < 0.01 vs. 0h of the same genotype; gp < 0.005 vs. 0h of the same genotype; hp < 0.001 vs. 0h of the same genotype.