Table 2. Polyamine content and epidermal thickness in TPA-treated skin from wild type and CAG-SpmS mice.
| Tissue | Treatment | Genotype | Polyamine content (pmol/mg tissue) |
|
Thickness (µm) | ||
|---|---|---|---|---|---|---|---|
| Putrescine | Spermidine | Spermine | Spm:Spd ratio | ||||
| Epidermis |
Acetone |
Wild type |
34 ± 25 |
163 ± 67 |
82 ± 20 |
0.53 ± 0.09 |
8.5 ± 0.4 |
| CAG-SpmS |
14 ± 6 |
72 ± 17 |
116 ± 32 |
1.60 ± 0.09c |
10.4 ± 1.3a |
||
| |
TPA |
Wild type |
48 ± 19 |
392 ± 51 |
110 ± 30 |
0.28 ± 0.06 |
43.4 ± 7.8 |
| CAG-SpmS |
82 ± 35 |
279 ± 27b |
253 ± 73b |
0.90 ± 0.20c |
42.3 ± 9.4 |
||
| |
|
|
|
|
|
|
|
| Dermis |
Acetone |
Wild type |
67 ± 37 |
195 ± 56 |
82 ± 16 |
0.43 ± 0.07 |
|
| |
CAG-SpmS |
26 ± 12 |
87 ± 13a |
99 ± 11 |
1.14 ± 0.07c |
|
|
| TPA |
Wild type |
60 ± 37 |
350 ± 54 |
103 ± 24 |
0.29 ± 0.03 |
|
|
| CAG-SpmS | 81 ± 59 | 187 ± 41c | 162 ± 45a | 0.86 ± 0.11c | |||
Polyamine content was determined in 7-week-old mice treated four times with acetone (n = 3 per genotype) or 17 nmol TPA (n = 5 per genotype). Skin was harvested 24h after the final treatment and processed for polyamine measurement or epidermal thickness (mean ± S.D.). ap < 0.05, bp < 0.005, cp < 0.001 vs. wild type controls.