Table 1. Pre-treatment of GBM6 cells with inhibitors of MEK1/2 and CHK1 causes radiosensitization. GBM6 cells, plated as single individual cells in sextuplicate, were treated with AZD6244 (500 nM); AZD7762 (100 nM) or the drug combination for 48h. The drug containing media was removed and fresh media lacking drugs added to the cultures. Cells were then irradiated (1 Gy, 2 Gy) and colonies permitted to form over the following 14 d. Data are the means of all conditions from multiple experiments (n = 3, ± SEM). #p < 0.05 less than CHK1 inhibitor value.
| VEH | AZD7762 | AZD6244 | AZD6244+7762 | |
|---|---|---|---|---|
| 0Gy |
100 |
64.3 (100) |
89.8 (100) |
36.4 (100) |
| 1Gy |
90.6 |
44.6 (69.3) |
78.6 (87.5) |
15.9 (43.6#) |
| 2Gy | 77.2 | 26.1 (40.5) |
57.3 (63.8) |
4.7 (12.9#) |