Figure 4.

Cell adhesion in Dictyostelium amobae. A) Cell-cell adhesion in the presence of 2 mM adenosine or 2 mM caffeine. It was carried out by scoring for the presence of single cells or clumps of cells with two or more cells adhered to each other. All values represent the mean ± standard error of 7 independent experiments. B) Expression pattern of Cad-1 proteins in AX2 cells. The Cad-1 expression level was checked in cells developed for 2 h and 4 h in Sorensen buffer in the presence or absence of either caffeine or adenosine. C) Expression levels of Cad-1 and CsaA in AX2 cells developed for 6 h, 8 h, 10 h, 12 h and 15 h in the presence or absence of either caffeine or adenosine.