Figure 5.

Cells grown in the presence of caffeine led to an increase in the levels of intracellular glucose or developing cells with extracellular glucose led to altered aggregate size and cellular adhesion protein Cad-1 and CsaA expressions: A) Effect of extracellular glucose on aggregate size. B) Cytosolic glucose levels of vegetative and developing cells in the presence or absence of either caffeine or adenosine. The glucose level was estimated as described in materials and methods. The values represent mean ± standard deviation (n = 8), *P < 0.1, **P < 0.01 (Student's t-test). C) Aggregates formed from cells grown in the HL5 medium in the presence or absence of caffeine for the 24 hours and subsequently harvested, washed and developed in the non nutrient agar in the absence of caffeine. D) Cad-1 expression levels in AX2 cells developed for 1 h, 2 h, 3 h and 4 h in Sorensen buffer in the presence or absence of 10 mM glucose. E) CsaA expression levels in cells developed for 6 h, 7 h, 8 h and 10 h in Sorensen buffer in the presence or absence of 10 mM glucose. F) CsaA expression levels in cells developed for 6 h in Sorensen buffer in the presence of different concentrations of glucose. G) Expression pattern of Cad-1 proteins in AX2 cells. Cells were grown in the presence or absence of 3 mM caffeine for 24 hours and Cad-1 expression levels were checked in cells developed for 1 h and 3 h in Sorensen buffer in the absence of caffeine. H) CsaA levels in AX2 cells grown in the presence or absence of caffeine. CsaA levels were checked in the cells developed for 1 h, 3 h and 5 h in Sorensen buffer in the absence of caffeine.