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. 2012 Mar 21;12:9. doi: 10.1186/1472-6750-12-9

Figure 2.

Figure 2

PCR analysis for detection of site-specific recombination and the recombinase transgene in the retransformation (TR1) and back cross (BC1) generations. PCR reactions (a, c) with primers e and f (Figure 1) or (b, d) with primers g and h (Figure 1) on representative plant DNAs. a, b) retransformed TR1-Bxb1.9.17 lines. c, d) Back crossed line BC1-Bxb1.28.6.3. Control lanes are B (blank, no DNA); E (excision, pN6-Bxb1exc DNA); N (no excision, pN6-Bxb1); P (pCOXS3-Bxb1). c) Lane numbers underlined indicate presence of excision event in the absence for the Bxb1 gene.