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. 2012 Mar 21;12:9. doi: 10.1186/1472-6750-12-9

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Copyright ©2012 Thomson et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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PCR analysis for site-specific recombination and the presence of the recombinase gene in the selfed (S₁) generation. PCR reactions (a) with primers e and f or (b) with primers g and h on representative plant DNAs. a, b) Selfed lines (lanes 1, 2) S₁-Bxb1.9; (lanes 3, 4) S₁-Bxb1.12; (lane 5, 6) S₁-Bxb1.28; (lane 7, 8) S₁-Bxb1.35 positive for excised target but the Bxb1 transgene is absent. Control lanes are B (blank, no DNA); E (excision, pN6-Bxb1_excDNA); N (no excision, pN6-Bxb1); P (pCOXS3-Bxb1).