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S1 plants examined by Southern blot analysis and whole plant genomic PCR. (a) Genomic DNA cleaved with EcoRV hybridized with a 32P-labeled GUS1350 probe (Figure 1). (b) Genomic DNA PCR amplified with with primers e and f. Plant lines (lanes 1, 2) S1-Bxb1.9; (lanes 3, 4) S1-Bxb1.12; (lane 5, 6) S1-Bxb1.28; (lane 7, 8) S1-Bxb1.35. Control lanes are wt (wild type Arabidopsis DNA) and TA3-Bxb1.9, (target lines).
