Table 1.

PCR analysis of TR₁ plants

TA Parent line	Plants tested	Positive for recombinase gene a and target locus b	Positive for excision c	Positive for excision and negative for unexcised productd
Bxb1.9	48	28	28	17
Bxb1.12	78	31	28	26
Bxb1.28	18	18	18	16
Bxb1.35	44	28	26	13

^a Primers g and h yielded the 0.72 kb Bxb1 fragment

^b Primers e and f yielded the 1.23 and/or 0.43 kb fragment

^c Primers e and f yielded the 0.43 kb excision fragment

^d Primers e and f failed to detect a 1.23 kb target fragment