Table 2.
PCR analysis of BC1 and S1 plants
| TR1-Parent line | Plants tested | Positive for target locus a | Positive for excision b | Positive for excision and negative for recombinase gene c | Positive for recombinase gene and negative for target locus d |
|---|---|---|---|---|---|
| Bxb1.9.7 | 48 | 36 | 20 | 0 | 1 |
| Bxb1.9.8 | 18 | 14 | 14 | 5 | 0 |
| Bxb1.9.11 | 6 | 6 | 6 | 0 | 0 |
| Bxb1.9.17 | 40 | 32 | 32 | 15 | 1 |
| Bxb1.9.23 | 51 | 41 | 36 | 0 | 9 |
| Bxb1.9.26 | 49 | 46 | 22 | 0 | 0 |
| Bxb1.9.29 | 18 | 16 | 12 | 0 | 1 |
| Bxb1.9.31 | 44 | 10 | 9 | 0 | 24 |
| Bxb1.9.44 | 32 | 28 | 26 | 17 | 0 |
| Bxb1.12.52 | 27 | 14 | 8 | 0 | 6 |
| Bxb1.12.61 | 9 | 6 | 6 | 1 | 2 |
| Bxb1.12.69 | 8 | 2 | 2 | 0 | 3 |
| Bxb1.12.72 | 9 | 4 | 1 | 0 | 3 |
| Bxb1.12.73 | 9 | 3 | 3 | 1 | 6 |
| Bxb1.12.74 | 9 | 9 | 4 | 0 | 0 |
| Bxb1.12.79 | 9 | 8 | 6 | 1 | 0 |
| Bxb1.12.81 | 9 | 7 | 3 | 0 | 0 |
| Bxb1.12.82 | 9 | 8 | 7 | 0 | 1 |
| Bxb1.12.83 | 9 | 9 | 3 | 1 | 0 |
| Bxb1.28.1 | 27 | 26 | 22 | 6 | 0 |
| Bxb1.28.2 | 18 | 18 | 14 | 0 | 0 |
| Bxb1.28.3 | 16 | 16 | 11 | 1 | 0 |
| Bxb1.28.4 | 9 | 8 | 8 | 3 | 0 |
| Bxb1.28.6 | 26 | 24 | 24 | 11 | 0 |
| Bxb1.28.7 | 17 | 15 | 13 | 0 | 0 |
| Bxb1.28.8 | 3 | 3 | 3 | 2 | 0 |
| Bxb1.28.9 | 18 | 17 | 17 | 4 | 0 |
| Bxb1.28.10 | 23 | 22 | 16 | 3 | 0 |
| Bxb1.28.11 | 8 | 7 | 7 | 2 | 0 |
| Bxb1.35.1 | 18 | 9 | 5 | 0 | 0 |
| Bxb1.35.2 | 36 | 28 | 17 | 7 | 0 |
| Bxb1.35.3 | 103 | 47 | 27 | 1 | 9 |
| Bxb1.35.15 | 23 | 19 | 14 | 5 | 0 |
| Bxb1.35.29 | 24 | 23 | 23 | 8 | 0 |
| Bxb1.35.32 | 23 | 23 | 23 | 0 | 0 |
| Bxb1.35.33 | 23 | 19 | 16 | 1 | 0 |
| Bxb1.35.36 | 24 | 23 | 23 | 5 | 0 |
| Bxb1.35.41 | 15 | 14 | 9 | 0 | 0 |
| Bxb1.35.44 | 17 | 17 | 16 | 0 | 0 |
a Primers e and f yielded the 1.23 and/or 0.43 kb target fragment
b Primers e and f yielded the 0.43 kb excision fragment
c Primers g and h failed to detect the 0.72 kb Bxb1 fragment
d Primers g and h yielded the 0.72 kb Bxb1 fragment and primers e and f failed to detect the 1.23 and/or 0.43 kb target fragment