Table 3.

PCR analysis of MC₁ plants

MC1-Parent line	Plants tested	Positive for target locus a	Positive for recombinase gene b	Positive for excision and recombinase genec	Positive for excision and negative for unexcised product d
Bxb1.9.17	31	30	29	28	28
Bxb1.9.31	31	31	19	19	19
Bxb1.12.69	16	16	14	14	14
Bxb1.12.72	16	15	7	7	3
Bxb1.35.15	28	26	25	24	24
Bxb1.35.33	46	45	36	35	13

^a Primers e and f yielded the 1.23 and/or 0.43 kb target fragment

^b Primers g and h yielded the 0.72 kb Bxb1 fragment

^c Primers e and f yielded the 0.43 kb excision fragment and primers g and h detect the 0.72 kb Bxb1 fragment

^d Primers e and f yielded the 0.43 kb excision fragment and failed to detect a 1.23 kb target fragment