Figure 2. Production of insulin protein by NPSC transduced with adenoviral vector carrying furin modified human insulin cDNA. (A and B) NPSC were cultured overnight as a monolayer on chamber slides, transduced with Ad-CMV-HI vector at a MOI of 0 (A) and 100 (B). Slides were collected after 24hrs, fixed with 1% paraformaldehyde and immunostained for insulin (A and B). Nontransduced SC do not express insulin (A), while transduced SC express insulin (brown, B).Twenty million NPSC transduced with Ad-CMV-HI vector (MOI 100) were transplanted into the renal subcapsular space of diabetic SCID mice. Graft bearing kidneys were collected at days 1 (C and D) and 10 (E and F) post-transplantation and immunostained for NPSC marker, vimentin (antibody does not cross react with mouse tissue) (brown, C and E) or insulin (brown, D and F). All the sections were counterstained with hematoxylin (blue).