Table 2. Johnsen’s score, number of proliferating and apoptotic germ cells per tubule, inhibin B and testosterone levels of control (C), heat-treated (H) and heat-treated plus electroacupuncture (H+EA) rats on day 37, 46, 56 and 79.
| Time (Day) | Symbol | Johnsen's score | Number of PCNA positive cells per tubule | Number of TUNEL positive cells per tubule | Inhibin B (pg/ml) | Testosterone (ng/ml) |
|---|---|---|---|---|---|---|
| Day 0 |
C0 |
10 (10–10) |
22.8 ± 3.1 |
0.2 (0.2–0.2) |
29.75 ± 2.31 |
2.12 (1.60–4.00) |
| Day 1 |
H1 |
9.3 (9.1–9.4) |
25.8 ± 3.4 |
63.7 (59.1–66.7)* |
18.88 ± 1.13* |
1.89 (1.31–4.38) |
| Day 9 |
H9 |
4.1 (4–4.2)* |
18.3 ± 0.8 |
0.7 (0.4–1) |
3.93 ± 2.52* |
2.88 (2.26–6.85) |
| Day 25 |
H25 |
3.7 (3.1–5.2)* |
6.8 ± 2.1* |
0.2 (0–0.6) |
2.71 ± 1.66* |
2.71 (2.59–4.38) |
| Day 37 |
C37 |
10.0 (9.8–10) a |
22.9 ± 2.4 a |
0.1 (0.1–0.2) |
27.94 ± 4.16a |
2.19 (1.07–6.86) |
| |
H37 |
5.3 (2.9–7.8) b |
11.2 ± 2.8 b |
0.5 (0–0.7) |
8.52 ± 4.69b |
3.50 (1.53–8.75) |
| |
H+EA37 |
6.7 (4.6–8.1) a,b |
22.7 ± 0.5 a |
0.4 (0.2–0.8) |
20.29 ± 2.88a,b |
2.72 (1.02–4.64) |
| Day 46 |
C46 |
10.0 (9.9–10) a |
20.3 ± 1.4 a |
0.2 (0.2–0.2) |
30.38 ± 3.21a |
4.31 (3.43–5.98) |
| |
H46 |
4.4 (3–8.6) b |
8.6 ± 2.6 b |
0.6 (0.3–1.2) |
9.53 ± 3.59 b |
4.98 (1.55–5.48) |
| |
H+EA46 |
7.7 (6.2–8.9) a,b |
21.2 ± 2.1 a |
0.5 (0.3–0.8) |
19.67 ± 2.26a |
3.48 (1.65–15.00) |
| Day 56 |
C56 |
10.0 (9.9–10) a |
25.3 ± 1.1 |
0.3 (0.3–0.3) |
27.98 ± 5.37 |
4.10 (2.62–20.38) |
| |
H56 |
7.0 (2.9–9.2) b |
14.6 ± 4.3 |
0.4 (0.3–3.4) |
13.79 ± 3.46 |
6.28 (2.51–13.42) |
| |
H+EA56 |
8.2 (3.5–9.3) a,b |
17.5 ± 4.1 |
0.4 (0.2–0.8) |
17.44 ± 0.76 |
6.85 (5.11–18.75) |
| Day 79 |
C79 |
10.0 (10–10) |
25.4 ± 1.1 |
0.3 (0.1–0.3) |
31.77 ± 4.64 |
3.20 (2.11–8.93) |
| |
H79 |
7.6 (3.2–9.4) |
17.4 ± 3.7 |
0.5 (0.4–1.1) |
24.07 ± 4.18 |
2.41 (1.54–13.69) |
| H+EA79 | 8.9 (2.7–9.8) | 16.0 ± 4.0 | 0.2 (0.2–0.3) | 20.24 ± 8.97 | 5.81 (1.40–23.06) |
Results were expressed as mean ± SEM for normally distributed data and median (range) for skewed data. a-b is significantly different (p < 0.05) between treatment groups of the same day. * is significantly different (p < 0.05) from C0.