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SDS/PAGE of refolded I domains. Purified wild-type (lanes 1 and 2), open (lanes 3 and 4), and closed (lane 5 and 6) I domains (2.5 μg) treated in sample buffer containing 10 mM DTT (lanes 1, 3, and 5) or 10 mM iodoacetamide (lanes 2, 4, and 6) were subjected to SDS/18% PAGE and Coomassie blue staining.
