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I domain binding to ICAM-1 in BIAcore. (A) Overlay of representative sensorgrams recording interaction of I domains with ICAM-1. Soluble I domains (1 μM) were interacted with the extracellular fragment of ICAM-1 (46) immobilized on the sensorchip. (B) Overlay of sensorgrams of different concentrations of the open I domain in the presence of 1 mM MgCl2.
