Table 2.

Kinetics of interaction of designed open I domain with ligands

Immobilized ligand	Analyte	kon, M−1⋅s−1 × 10−5	koff, s−1 × 102	KD*, nM
Open I domain	sICAM-1	1.07  ± 0.03	3.16  ± 0.26	258  ± 24
sICAM-1	Open I domain	1.39  ± 0.08	2.49  ± 0.26	185  ± 12
ICAM-1-Fc	Open I domain	1.18  ± 0.14	2.14  ± 0.17	173  ± 26
ICAM-2-Fc	Open I domain	0.26  ± 0.02	1.97  ± 0.31	605  ± 55
ICAM-3-Fc	Open I domain	0.18  ± 0.02	6.44  ± 0.97	4,320  ± 202

^*K_D was measured as described in Table 1.