Table 3. Replication of rRVF mutant viruses in Culex quinquefasciatus mosquitoes.
| Dissemination rates | Transmission rates | ||||||||||||
| Infection rate1 | # pos/# exposed2 | # pos/# infected3 | # pos/# exposed4 | # pos/# disseminated5 | |||||||||
| Virus | Experiment | Bloodmeal titer6 | Avg (sd) day 0 titer7 | % positive (n) | % positive (n) | % positive (n) | % positive (n) | % positive (n) | |||||
| rRVF-wt | 3 | 7.9 | 4.3 (0.2) | 94.3 | (35) | 9.9 | (131) | 7.1 | (28) | 6.7 | (30) | 100.0 | (2) |
| rRVF-wt | 4 | 8.2 | 5.5 (0.4) | 95.7 | (25) | 16.0 | (25) | 16.7 | (24) | 4.0 | (25) | 25.0 | (4) |
| rRVF-ΔNSs | 3 | 8.5 | 5.8 (0.2) | 94.3 | (35) | 7.1 | (113) | 11.4 | (35) | 6.7 | (30) | 66.7 | (3) |
| rRVF-ΔNSm | 3 | 6.5 | 3.2 (0.2) | 11.4 | (35)* | 0.8 | (119)* | 25.0 | (4) | 0 | (30) | 0 | (1) |
| rRVF-ΔNSs-ΔNSm | 4 | 8.3 | 6.2 (0.2) | 66.0 | (50)* | 10.0 | (50) | 15.2 | (33) | 4.0 | (50) | 40.0 | (5) |
Number of mosquitoes containing detectable virus by plaque assay divided by number of mosquitoes exposed to infectious bloodmeal.
De = number of mosquitoes with RVFV antigen in head tissues divided by number of mosquitoes exposed to infectious bloodmeal.
Di = number of mosquitoes with RVFV antigen in head tissues divided by number of infected mosquitoes.
Te = number of mosquitoes with positive saliva by plaque assay divided by number of mosquitoes exposed to infectious bloodmeal.
Td = number of mosquitoes with positive saliva by plaque assay divided by number of mosquitoes with disseminated infection.
Titer expressed as log10 PFU/mL.
n = 3, titer expressed as log10 PFU/ml; avg is log10 geometric mean; sd is standard deviation.
*: value differs significantly from rRVF-wt values; see Table S2 for statistical analysis results.