Table 2. Descriptions of gene-specific real-time PCR assays.
| Gen Symbol | Primer Sequences (5′-3′) | Am (bp) | Tm (°C) | E (%) | Ref |
| Candidate reference genes for microRNA normalization | |||||
| 5S | RT: AGCCTACAGCACCCGGTATT | 40 | 81.6 | 94 | [30] |
| F: GCCCGATCTTGTCTGATCTC | |||||
| R: CCTGACCCTGCTTAGCTTCC | |||||
| miR-16 | RT: GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACCGCCAA | 50 | 83.5 | 91 | [31] |
| F: CAGCCTAGCAGCACGTAAAT | |||||
| R: GAGGTATTCGCACCAGAGGA | |||||
| miR-103 | RT: GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACTCATAG | 51 | 79.8 | 104 | [31] |
| F: TACGCAGCAGCATTGTACAG | |||||
| R: GAGGTATTCGCACCAGAGGA | |||||
| miR-191 | RT: GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACCAGCTG | 49 | 80.0 | 104 | [31] |
| F: CACCAACGGAATCCCAAA | |||||
| R: GAGGTATTCGCACCAGAGGA | |||||
| miR-Let7a | RT: GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACAACTAT | 50 | 81.5 | 96 | [31] |
| F: CGCGCTGAGGTAGTAGGTTG | |||||
| R: GAGGTATTCGCACCAGAGGA | |||||
| RNU48 | RT: GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACGGTCAG | 65 | 88.5 | 93 | [31] |
| F: TCTGAGTGTCTTCGCTGACG | |||||
| R: GAGGTATTCGCACCAGAGGA | |||||
| U6 | RT: AACGCTTCACGAATTTGCGT | 93 | 85.7 | 94 | [32] |
| F: CTCGCTTCGGCAGCACA | |||||
| R: AACGCTTCACGAATTTGCGT | |||||
| Candidate reference genes for mRNA normalization | |||||
| 18S | RT: GAGCTGGAATTACCGCGGCT | 159 | 87.8 | 97 | a |
| F: AAACGGCTACCACATCCAAG | |||||
| R: TTGCCCTCCAATGGATCCT | |||||
| ACTB | F: ATTGCTGACAGGATGCAGAA | 109 | 86.7 | 104 | [33] |
| R: TAGAGCCACCAATCCACACAG | |||||
| ALB | F: GATGCCGTGAAAGAGAAAGC | 196 | 89.2 | 93 | [34] |
| R: CGTGACAGCACTCCTTGTTG | |||||
| B2M | F: ACATCCTGGCTCACACTGAA | 109 | 87.1 | 94 | [35] |
| R: ATGTCTCGGTCCCAGGTG | |||||
| CYCA | F: AGCACTGGGGAGAAAGGATT | 248 | 87.8 | 97 | [36] |
| R: AGCCACTCAGTCTTGGCAGT | |||||
| GAPDH | F: GTATCGGACGCCTGGTTAC | 128 | 87.1 | 93 | a |
| R: CTTGCCGTGGGTAGAGTCAT | |||||
| HPRT1 | F: GCTGAAGATTTGGAAAAGGTG | 157 | 87.7 | 92 | [26] |
| R: AATCCAGCAGGTCAGCAAAG | |||||
| SDHA | F: AGACGTTTGACAGGGGAATG | 160 | 89.8 | 97 | [26] |
| R: TCATCAATCCGCACCTTGTA | |||||
| Genes of interest | |||||
| miR-122 | RT: GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACCAAACA | 50 | 83.4 | 97 | [31] |
| F: GGCTGTGGAGTGTGACAATG | |||||
| R: GAGGTATTCGCACCAGAGGA | |||||
| CCNG1 | F: AGTCTTAAGGGACGTCAGGAG | 119 | 86.1 | 92 | a |
| R: GCTGAGGAGCTACCCACATT | |||||
Am: amplicon size. bp: numer of base pairs. E: Assay efficiency. Tm: melting temperature. Ref: references. RT: retro-transcription primer. R: Reverse primer. F: Forward primer. a: Primers were designed using Primer3 software.