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. 2012 May 1;7(5):e35865. doi: 10.1371/journal.pone.0035865

Figure 2. RT-PCR and immunoblot analysis of Rpgr expression in mutant and wild-type mice.

Figure 2

A) Schematic of the mouse Rpgr gene showing the locations of the ORF15 exon, the oligonucleotide primers for RT-PCR, and protein sequences used to generate the RPGR antibodies S1 and S3. B) RT-PCR was performed on total retinal RNA from two-month-old male mice using a common exon 1 primer and reverse primers for ORF15 or exon 19. The PCR products were resolved on a 1% agarose gel. RT-PCR of Hprt was used as a control for RNA recovery and loading. C) Western blots of retinal extracts from 2-6 month-old male B6 (wild type), Rd9, and Rpgr-KO mice probed with RPGR antibodies. The S1 antibody recognizes a sequence common to both Rpgr ORF15 and 1–19 variants. The S3 antibody recognizes only the protein corresponding to the 1–19 variant.