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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Free Radic Biol Med. 2012 Feb 25;52(9):1744–1749. doi: 10.1016/j.freeradbiomed.2012.02.021

Table 1.

Yield of cytosolic proteins, total nDNA, and mtDNA isolated simultaneously from wildtype and Sod2−/+ fibroblasts.

Protein/Cell [pg] nDNA/Cell [pg] mtDNA/Cell [pg]
Sod2+/+ 141.11 ± 7.30 4.94 ± 1.91 0.018 ± 0.008
Sod2−/+ 145.23 ± 68.26 3.76 ± 1.13 0.034 ± 0.009

Protein yield was measured using the DC protein assay. DNA concentration was determined by absorption at 260 nm. Cells were counted using a Neubauer counting chamber after trypan blue staining. At least three isolation procedures were performed for one data point. Each data point represents the mean value ± standard deviation.