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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Free Radic Biol Med. 2012 Mar 9;52(9):1760–1766. doi: 10.1016/j.freeradbiomed.2012.02.052

Fig. 2. Silencing of Nox1 expression by siRNA inhibits monocyte adhesion to VSMC induced by IL-1β and U46619.

Fig. 2

Rat VSMCs transfected with siRNA-Control or siRNA-Nox1 were treated for 24 hours as indicated. A, RT-PCR and B, Real-time RT-PCR show siRNA silencing of Nox1 expression. C, Monocyte adhesion. THP-1 monocytes were co-cultured for 3 hours with VSMCs that had been treated for 24 hours as indicated, and then unbound monocytes were washed away. Bar graphs show numbers of monocytes bound to VSMC per area and represent data from 6 separate experiments.