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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Free Radic Biol Med. 2012 Mar 9;52(9):1760–1766. doi: 10.1016/j.freeradbiomed.2012.02.052

Fig. 3. Silencing of Nox1 expression does not affect VCAM-1 or iNOS expression.

Fig. 3

Rat VSMCs transfected with control or Nox1 siRNA were treated for 24 hours as indicated. A, Western blot analysis of VCAM-1 and iNOS protein expression. Total ERK levels are shown as a reference for equal loading. B and C, Real-time RT-PCR analysis of VCAM-1 and iNOS mRNA levels. Bar graphs represent 3 separate experiments. The results were normalized to GAPDH mRNA levels and expressed as fold-changes compared to IL-1β-treated cells (column 2).