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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Biomaterials. 2012 Apr 9;33(20):5166–5174. doi: 10.1016/j.biomaterials.2012.03.054

Fig. 5.

Fig. 5

PEC cells co-treated with M13-RGD8-PmpD3 and Ct show trafficking of phage into the inclusion. (A) Timeline from 12 h to 23 h; PEC cells infected with M13-RGD8-PmpD3 at 12 h; inclusion surrounded by phage at 20 h; invasion of the inclusion by M13-RGD8-PmpD3 begins at 24 h and 30 h; disruption of inclusion and dispersal of contents at 36 h. (B) Same timeline as in A except there is no infection with M13-RGD8-PmpD3; the inclusions are visibly larger than in A throughout the developmental cycle of the organism. (C and D) Laser scanning microscopy with orthogonal and 3D projection of Z-stack (x–z and y–z plane) imaging using a Zeiss LSM 710 confocal microscope; height of field displays an orthogonal slice through a 3D grid showing M13-RGD8-PmpD3 inside the inclusion. (E) laser scanning microscopy with orthogonal Z-stack (x–z and y–z plane) imaging using a Zeiss LSM 710 confocal microscope; height of field displays an orthogonal slice through a 3D grid showing that M13-RGD8 does not translocate into the lumen of the inclusion. Data represent three independent experiments.