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(a) Positive immunohistochemistry for PTEN. Diffuse cytoplasmic staining is present in the majority (>90%) of tumor cells. (b) Negative immunohistochemistry for PTEN. No or only scattered tumor cells (<1%) have cytoplasmic staining. Note stromal cells serve as positive internal control. (c) Heterogeneous immunohistochemistry score for PTEN. Heterogeneous tumors have distinct positive and negative foci. All photomicrographs 20×.
