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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Mol Immunol. 2012 Apr 7;51(2):234–244. doi: 10.1016/j.molimm.2012.03.025

Fig. 4. Alanine/Serine scan of p35 subunit and contribution to IL-12 and IL-35 formation.

Fig. 4

Lysates from HEK293T cells transfected with wild type or mutant p35 and either (A) p40 or (B) Ebi3 were assessed for cytokine formation by immunoprecipitation and western blot. Lysates were immunoblotted for GFP as a loading control. (C) Western blots from at least 3 independent experiments were quantified and plotted as percent of wt IL-12 or IL-35 (mean ± SEM). *p< 0.05, ** p< 0.01, *** p< 0.001