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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Mol Immunol. 2012 Apr 7;51(2):234–244. doi: 10.1016/j.molimm.2012.03.025

Fig. 5. Alanine/Serine scan of Ebi3 subunit and contribution to IL-27 and IL-35 formation.

Fig. 5

Lysates from HEK293T cells transfected with wild type or mutant Ebi3 and either (A) p28 or (B) p35 were assessed for cytokine formation by immunoprecipitation and western blot. Lysates were immunoblotted for GFP as a loading control. (C) Western blots from at least 3 independent experiments were quantified and plotted as percent of wt IL-27 or IL-35 (mean ± SEM) * p<0.05, ** p<0.01